Abstarct: For optimizing tissue culture of Prunus armeniaca, a study was carried out by several factorial experiments on the baxse of completely randomized design. In the first and second experiments, the effect of different concentrations of 6-benzylaminopurin (BAP) in combination with different concentrations of indole-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA) on basal MS medium were evaluated for callus induction from leaf explants. The results showed that the highest callogenesis percentage (86% in L.cv “RajabAli”) obtain on MS medium supplemented with 2/2 μM BAP and 5/36 μM NAA. In the third experiment, The effects of explant types and different concentrations of plant growth regulators was evaluated on callus induction. Highest rate of callus induction ,90%, observe in vitro-derived leave explants which cultured on MS medium with 2/2 μM BAP and 2/68 μM NAA. In the next experiment, The effects of plant growth regulator treatments, light conditions and explant types was studied on adventitious shoot regeneration. Results showed that the highest shoot regeneration on cotyledon and hypocotyl explants ,51% and 48%, obtain in 9 μM BAP plus 4/5 μM KIN and 5/5 μM NAA and 7/5 μM TDZ plus 2/5 μM IBA, respectively.The dark incubation especially in cotyledon explants was effective in increasing the regeneration frequency. In further experiments, the effect of different basal mediums was analyzed on regeneration rate of cotyledon and hypocotyl explants, separately. The results showed MS and QL basal salts was more effective on shoot regeneration from hypocotyl (50%) and cotyledon (83%) explants, respectively