Abstarct: Bacterial phytopathogens have a great impact on yield loss. In between, the causative agent of citrus canker Xanthomonas citri subsp. citri (Xcc), has influenced citri culture worldwide. Use of chemicals, the common method of bacterial control, has many negative effects on ecosystems. On the other hand, biological control of bacterial pathogens is an envirmentally frindly technique that was considered in this study. Two fungal secretomes were used to study their antagonistic effects against an isolate of Xcc, namely NIGEB-88 with disk diffusion method. In order to determine the nature of antimicrobial agent, present in both sectetoms, they were treated with a range of protease. As a result the antimicrobial activity were diminished significantly that suggesting being a protein by nature. In continuation of our studies the The inhibitory spectrum of antimicrobial proteins (AMPs) were tested against available bacterial and fungal species, that could inhibit their growth. These compounds showed to be tolerant in response to heat, basic and acidic pHs and number of detergents, too.