Abstarct: This research was performed in the research farm of sabzevar agriculture burean research center in crop year 1391 to study the effect of different cultivation methods on weed seed distribution.to describe patterns of spatial and density of weeds, Amaranthus retroflexus, Chenopodium album L, Echinochloa crus – galli L and Polygonum aviculare L. as the most important annual weeds common in the experiment field Geostatistical methods were used. Systematic sampling method was done, sampling at 6 meters x 6 meters and boxes 0/5 mm x 0/5 mm, two-stage sampling from the seed bank of weeds before plowing and weed seedling counts after applying the methods of tillage perpendicular to the disk twice, once disc, cultivator and moldboard. Continuity of spatial changes of the variables was plotted, using variogram functions and distribution maps of species distribution. Variograms showed that twice perpendicular disc tillage method, caused the horizontal movement of seeds in the soil for plowing, while once disc tillage method, caused dispersion of weed seeds all over the field and tillage using Moldboard plow made seeds move vertically deep in the soil. Using tillage cultivator had no effect on the movement of weed seeds. The minimum spatial correlation between seed bank of weeds and weed seedlings was related to once disc tillage method while the maximum spatial correlation was related to Moldboard plow tillage. Spatial distribution plans confirmed spot distribution of weed. Having any information about the distribution of weeds in the fields can be helpful in improving of making decisions like tillage methods, use of herbicides, selecting the herbicide type or applying the amount of herbicide also haring such information can be helpful in designing weed control programs. Also having such information can be helpful in designing weed control programs.