Abstarct: A factorial experiment in a randomized complete block design with four replications was conducted in Shahrood University in 1391 to assess the effect of priming on the field and inoculated with Thiobacillus bacteria and different levels of sulfur on yield and yield components of soybean. Factors include priming, Thiobacillus bacteria and organic granular sulfur. Priming at two levels: non-prime and prime in farm, Bacteria in two levels: consumption and non-consumption and organic granular sulfur in three levels: Non consumption, consumption of 150 kg per ha (recommended), 225 kg per ha (1.5 times more than recommended). Results showed that main effect of priming, Thiobacillus bacteria and organic granular sulfur had significant impact on number of pods per plant, grain weight, biological yield, grain yield, plant height, oil percentage, protein percentage and grain sulfur. The interaction of Thiobacillus bacteria and organic granular sulfur showed significant impact on grain phosphorous content and the amount of chlorophyll b on 1% level. The overall conclusion from this experiment confirms that priming technic, Thiobacillus bacteria and organic granular sulfur increase the growth and yield of soybean and better establishment of plants. Consumption of Thiobacillus bacteria and organic granular sulfur also reduce soil pH and improve chemical properties of the soil.