Abstarct: In this proposed a simple and powerful microextraction technique was used for detection of copper at ultratrace levels in water samples using Dispersive Liquid Liquid Microextraction (DLLME), followed by flame atomic absorption spectrometry (FAAS). In this technique, a Homogeneous mixture of acetone (disperser solvent) and chloroform (extraction solvent), was rapidly injected into the aquoes sample containing copper and salophen. A cloudy solution (water, acetone and chloroform) was formed. In this process, the complex Cu(II)-salophen were extracted into the fine droplets of CHCl3. After centrifugation, the sedimented phase (CHCl3) was diluted by ethanol and for determination was spray into flame of FAAS. All of parameters affecting in DLLME was optimized. Moreover, the effects of the interfering ions on the analytes recovery were also investigated. Under the optimum conditions, the enrichment factor of 48 was obtained from only a 10.0 mL aquoes sample. The calibration graph was linear in the range of 3.0-120µg.L-1 with the detection limit of 0.6µg.L-1. The relative standard deviation (RSD) for five replicate measurements of 5.0, 20 and 50µg.L-1 of copper was 4.1%, 1.48% and 1.8%, respectierly. The proposed method was used for analysis of different water sample and a rice sample with good accuracy.