Abstarct: In this research, pure and doped WO3-NPs with Ag, Zn, and Se (3, 5, and 7%) were prepared by green chemistry method in gelatin and chitosan substrates from Na2WO4. 2H2O as a precursor. In these processes, the organic molecules of substrates, as a capping agent, prevent the growth and agglomeration of WO3-NPs. Optical, physical, and chemical properties of synthesized nanopowders were identified using FT-IR, UV-Vis-DRS, FESEM, EDX, RAMAN, ICP, XPS, PXRD, pHpZC, and TGA/DTA analyses. The results showed that the substrates and dopants character have direct effects on morphology, crystal size and phase, and bond gap energy of the produced samples. Therefore, the produced pure and doped WO3-NPs showed different activity in organic dyes degradation, anticancer, and antibacterial properties. Using different scavengers showed that OH° and O°2- are the main active species produced in the photocatalytic remediation (96-98%) of MB, Rh-B, and MO dyes under UV light (250 nm, 20 W) irradiation. Also, the results showed that all produced photocatalysts could be recovered 5 times with only 8-9% fall in their activity. The MTT test on cell lines Huh-7, B16F0, 4T1, and LnCap confirmed that doped nanoparticles with Ag, Zn, and Se have a higher toxicity than the pure WO3-NPs. The antibacterial properties of nanoparticles were also investigated using a well-diffusion method on gram-positive (S. aureus) and gram-negative (K. pneumoniae) bacteria. The results showed that the antibacterial properties of these nanoparticles at concentrations of 1000 µg/mL are more effective on Klebsiella bacteria.