Abstarct: In this study a highly simple and sensitive spectrometric method for determination of copper (ІІ) is proposed. In this investigation, neocuproine hydrocholoride (NC) was used as complexing agent and new preconcentration method termed directly suspended droplet solvent microextraction (DSDME), was developed. In this technique a free microdroplet of solvent is delivered to the surface of an immisible aqueos sample while being agitated by a stirring bar placed on the bottom of the sample cell and creating vortex at the center of the vial, also solvent is placed at the bottom of the vortex. when extraction is completed, a portion of the organic droplet is sampled with a microsyring and it‘s copper content determined by flame atomic absorption spectrometry. Some effective parameters on extaction and complex formation, such as pH, the kind of extraction solvent, solvent volume, extraction time, stirring rate, were optimized. Under the optimum conditions, the enrichment factor of 26.37 and recovery 98.06 was obtained. The calibration curve was linear in the range of 0.008-0.24 mg/L with detection limit of 0.83µg/L. The relative standard deviation for six determination of concentration (0.008, 0.72, 0.144 mg/L) of copper was (6.13%, 3.69%, 2.78%) respectively. As a result, this method can be successfully applied for the determination of copper in different samples.