Abstarct: The preparation of new targeted drugs that are effective only in the desired location, and do not damage healthy tissues, is always the subject of new research. In this regard, functionalized polysiloxane was investigated for drug loading in clinical activities in this study. Thus, the polysilsesquioxane substrate was prepared by sol-gel method at room temperature and it was characterized by the Field Emission-scanning electron microscope (FE-SEM), Fourier transform infrared spectrometry (FT-IR), UV–visible diffusive reflectance spectrometry (DRS), and other methods. Subsequently, crocetin which is used in the treatment of dermal diseases, was stabilized as a target drug on polysiloxane substrate. In addition, the conditions of crocetin release and the therapeutic performance of the synthesized compound were also evaluated on a dermal wound model. The results showed that the synthesized compound had better effect on wound closure than the gauze-treated wound (the control group).