Abstarct: Pistachio as an important and strategic product plays an important role in non-oil export of the country. Hence, increasing the amount and quality of this product is important. Pestiside residues in pistachio treat human and environment health and also cause serious problems in exporting of this product. In this dissertation, the spirotetramat pesticide residue and its most important mextabolite, spirotetramat-enol, were evaluated using the QuChERS method, which is a fast, easy, inexpensive, safe and reliable method for complex tissues. To isolate and measure this pesticide, high perfomance liquid chromatography with UV/Vis detector was used. HPLC instrumental such as wavelength of analysis and solvent for preparation of standards were evaluad and optimized. The QuChERS method consists of two steps: 1) extraction by acetonitrile. 2) a cleaning step using salts and sorbents. The amount of graphene carbon black and the primary and secondry amines at the purification step as well as the amount of water at the extraction stage were optimized. Finally, the method was evaluated for linearty range, detection limit according to the maximum residual value of pesticidein Iran, and also recovery and repeatability. The matrix-match calibration curve for spirotetramat showed a linear relation in the range 0.040-1.0 mg/ L with correlation coefficient of 0.9978. The RSD was less than 10% and the recovery range was 91-103%. In addition, the matrix-match calibration curve for spirotetramate-enol exhibited a dynamic range of 0.050-1.0 mg/L with correlation coefficient of 0.9936. The LOD and LOQ for spirotetramate was 2.2 µg/kg and 0.053 mg /L, respectively which is acceptable according to the MRL of this pesticide (50 µg/kg).