Abstarct: Since the Pd and Pt complexes are suggested to be more effective than the other anticancer drugs, we decided to prepare some new water soluble platinum(II) and palladium(II) complexes. First we prepared two complexes with general formula [M(DACH)(FIP)](NO3)2 in which M=Pd(II) or Pt(II), DACH=(1R,2R)-(−)-1,2-Diaminocyclohexane, and FIP: 2-(furan-2-yl)-1H-imidazo[4,5-f][1,10]phenanthroline). The second and third series of the complexes with general formula [M(DACH)(L)]NO3 (where, M=Pd(II) or Pt(II), L= methyl-, butyl-, Isopentyl- and tert-pentyl glycine) were also prepared. These complexes have been characterized by spectroscopic methods such as ultraviolet-visible, infarared and 1H-NMR as well as conductivity measurements and chemical analysis. Anticancer activity of these compounds were tested against cancer colon cells of HCT116. The interaction of these complexes with calf thymus DNA (ct-DNA) was studied by using different techniques such as UV-Vis, fluorescence and circular dichroism as well as molecular dynamics (MD) simulation and molecular docking. By using denaturation data, the midpoint of titration, [L]1/2, and thermodynamic parameters have been determineded in tris buffer containing 10 mM sodium chloride (pH=7.4) at 27 and 37 °C. Moreover, the modes of binding of all complexes to ct-DNA were investigated by fluorescence spectroscopy and circular dichroism, which confirmed these synthesized complexes interact with DNA through non-covalent bonding modes. Data indicated [Pt(DACH)(FIP)](NO3)2 can denature DNA at the low concentration than the other synthesized complexes. Besides, Molecular docking results showed binding energy for this complex was more negative than other complexes (-13.2 kcal/mol) which revealed more effective DNA-binding affinity of this compound.