Abstarct: In this study ligand have been prepared from the reaction of 1,2-bis(2- aminothiophenyl) ethane with 2-hydroxy-3-methoxybenzaldehyde in ethanol solution, then Cu(II), Co(II), Fe(III) complexes of ligand have been synthesized in ethanol. Characterization of these ligand and its complexes have been carried out by conductivity measurements, IR, 1HNMR, 13CNMR and Mass spectrometry. These ligands as hexadentate containing atoms (N, S, and O), coordinate to the transition mextal ions and form stable complexes, then ligand and the mextal complexes were also tested for their in vitro antimicrobial activities to assess their inhibiting potential. Cu(II) and Fe(III) complexes were screened for their antimicrobial activity against E.amylovera, X. citri, E. coli and M. phaseolina .