Abstarct: In this research a simple and sensitive method of dispersive liquid-liquid microextraction (DLLME) was proposed for preconcentration of Copper prior to, it’s determination by flame atomic absorption spectrometry. The analytical procedure baxsed on the formation of a complex between Cu(ІІ) with N-Salicylideneaniline as a complexing agent. After extraction of the complex by carbon tetrachloride as extraction solvent and ethanol as disperser solvent, copper concentration in organic solvent drop was determined by flame atomic absorption spectrometry. The effect of different parameters such as pH, kind and volume of buffer, kind and volume of extraction solvent, kind and volume of disperser solvent, kind of dilution solvent, volume of aqueous phase, concentration of ligand, ionic strength, extraction time and centrifuge time were investigated. Also, the effects of foreign ions were investigated. Under the optimized conditions, a linear calibration curve in the range of 3.0-13.0 µg L-1, with detection limit of 0.60 µg L-1 and enrichment factor of 43.2 were achieved. The relative standard deviation for six replicate determinations of 0.020, 0.050 and 0.070 mg.L-1 of Copper (ІІ) were 2.54%, 1.38% and 1.14%, respectively. This method was successfully applied for determination of copper (ІІ) in the spiked and natural samples.