Abstarct: In this study we have studied morphological, structural, optical and photo-electrochemical properties of titanium dioxide (TiO2) nanostructures grown by chemical anodization on titanium foil. In order to characterize our samples we have used various apparatus including: Field Emission Scanning Electron Microscopy (FESEM), X-ray diffraction (XRD), photoluminance (PL) and the current-voltage (I-V) measurements. The studied samples in this research are prepared using ethylene glycol electrolyte solution together with ammonium fluoride (acid) in different conditions of applied voltage, temperature of electrolyte solution, concentration of water in electrolyte and annealing temperature. FESEM images indicate the formation of TiO2 nanotubes with different aspects of internal diameter, wall thickness and length in different growth conditions. X-ray diffraction spectra of the samples indicate the formation of samples in polycrystalline structure in anatse phase with (101) as the preferred orientation and in some instances of rutile phase with (110) as the main orientation. The PL spectra show emissions in the ultraviolet (band to band recombination), blue and green (crystal defect-related recombinations) regions. The photo-electrochemical properties of the samples (the I-V measurements) in dark and under ultraviolet irradiation show that not only the surface morphology but also the crystallinity of the sample affects on its light sensitivity.