Abstarct: In this research, we have investigated the surface morphology, structural, electrical and optical properties of nanostructured vanadium pentoxide (V2O5) thin films grown on glass substrate by spray pyrolysis method. For preparation of V2O5 thin films, we have used vanadium trichloride (VCl3) as the chemical composition. The variable parameters in this study are including: substrate temperature (section 4-2), annealing effect (section 4-3) and zinc doping (section 4-4). For characterization of the synthesized samples we have used various methods: Field Emission Scanning Electron Microscopy (FESEM), X-ray diffraction (XRD), Raman spectroscopy, Hall effect, and Ultraviolet-Visible (UV-Vis) spectroscopy. Through the study of substrate temperature, in the range of 350-450 C, it is found that with increasing the growth temperature the surface of the laxyers are covered with nanorods. XRD results showed that most of the synthesized laxyers have orthorhombic structure in V2O5 phase and in some cases are together with additional V4O9 crystalline phase. Also the characteristic peaks in Raman spectra indicated the formation of the V2O5 phase. Optical and electrical properties showed that with increasing the substrate temperature, the band gap of the laxyers had a decreasing trend and electrical conductivity of the samples had an increasing trend. For annealing process, annealing temperature (400 and 450 C) and time interval (2 and 3 h) have been investigated. It is found that annealing process has led not only to higher amounts of nanorods on the surface of the samples, but also has improved the crystalinity of the laxyers. Electrical properties of the samples showed that annealing at 450 C for 2h tend to increase the electrical resistance of the laxyer. In the study of the influence of doping of the sample by zinc (Zn) atoms, it was revealed that through this process the diameter of the nonorods are increased. Electrical and optical properties of the synthesized samples showed that with increasing the doping level, due to the role of zinc atoms as the acceptors in the crystalline lattice of vanadium oxide, the electrical resistance of the laxyers are increased and their band gaps are decreased.