Abstarct: In this research, we are investigated the surface morphology, structural, electrical, thermo-electrical and optical properties of nanostructured vanadium pentoxide (V2O5) thin films grown on glass substrate by spray pyrolysis technique for photoconduction properties application. For characterization of synthesized samples we have used various equipment including: Field Emission Scanning Electron Microscopy (FESEM), X-ray diffraction (XRD), Raman spectroscopy, current-voltage (I-V) and Seebeck effect measurements. For preparation of V2O5 thin films, we have used vanadium trichloride (VCl3) chemical composition. The variable parameters in this study are as following: spray rate (section 4-2), precursor volume (section 4-3), precursor concentration (4-4) and annealing effect (section 4-5). Depending on growth conditions, the FESEM images of the samples indicated the formation of relatively uniform nano grains, less than 100 nm in size, on the surface of the film. The XRD results showed that most of the synthesized laxyers have orthorhombic structure in V2O5 phase and in some cases with additional V3O7 and V4O9 crystalline phases. Also the characteristic peaks in Raman spectra indicated the formation of the V2O5 phase. The thermoelectric results (Seebeck effect) of the laxyers confirmed that all synthesized samples have n-type electrical conductivity. We also found that the annealing process in the sample with the highest solution concentration (90 mM) can improve the electrical and opto-electrical properties (photoconduction effect) of the sample compared to the as-grown one.