Abstarct: In this experimental investigation zinc oxide nanostructured thin films synthesized by spray pyrolysis method and were characterized by various physical properties including: morphological, structural, optical, electrical, thermoelectrical properties also their application in ultraviolet (UV) photoconductivity properties. In this study we have used the Field Emission Scanning Electron Microscopy (FESEM), X-ray diffractometer (XRD), UV-Vis spectrophotometer, I-V curve, and Seebeck effect measurements. In this research work we have studied the effect of: spray rate in pure and nitrogen doped zinc oxides (ZnO:N), nitrogen concentration and finally substrate (presence of a buffer laxyer) on physical properties of zinc oxide nanostructure thin films and their usage in UV photodetectors. We found all samples with n-type conductivity, are grown in nanostructure wurtzite phase polycrystalline nature with (002) and (101) as preferred orientations. It is shown that surface morphologies, crystallite sizes and electrical properties of the films have crucial effects on the ultraviolet photo-detections properties.