Abstarct: Blood flow restricted training useful and effective training exercises for increased is athletes performance level.The aim of this study was to evaluate the effect of acute on a treadmill training with BFR on serum levels of growth hormone (GH), insulin like growth factor 1 (IGF-1), cortisol and hemodynamic factors in inactive girls.Thirty healthy girls student with an age average of 20/27±1/76 years who volunteered to participate in the study were selected as samples and randomly divided into three groups: 1) training on a treadmill BFR (intensity: 2 miles/hour, 5 sets of 2 minutes with one minute of rest in between each set), 2) training on a treadmill without BFR (with the samevolume of BFR) and 3) control (BFR and without exercise). The level of hormones in fasting were measured before and immediately after exercise. The hemodynamic factors were measured before and 20 minutes after the exercise. Data were analyzed with one-way ANOVA in the level of p< 0.05. Data were analyzed showed serum GH , IGF-1 and cortisol levels significantly increased in post exercise in BFR group compared to pre-exercise (P<0.05). The results of one-way ANOVA showed Between Average serum GH , IGF-1 and cortisol levels Three groups after exercise a significant difference (P<0.05) As well as the results showed that both BFR and non-BFR exercise increased HR, SBP, RPP significantly in post exercise compared to pre-exercise (p˂0.05). After exercise, no significant differences in HR and SBP were observed between three groups (p˂0.05) and Increases in hemodynamic factors were greater in BFR group than without BFR group. In conclusion, acute walking on a treadmill with BFR could increases in anabolic and catabolic hormones and some changes in hemodynamic factors in inactive young girls. In the limited blood flow show that the heart muscle needs in Response to BFR exercises. So in terms of practical, seem limited blood flow in the training on a treadmill is an important factor in the creation of acute hormonal responses and there are executive limitations lower compared with high-intensity resistance training and realization the expected goals of the high-intensity training and is applicable for wider range of people, including inactived individuals, disabled, sick and elderly.