Abstarct: Diabetes is a major health problem worldwide. Exercise training and supplementation are effective approaches for improving the condition of diabetic individuals. This study aimed to investigate the effects of resistance training and synbiotics on arterial stiffness, nitric oxide, and glycated hemoglobin in postmenopausal women with type 2 diabetes.In this semi-experimental study, 45 eligible women were randomly assigned to three groups: resistance training + placebo (n=15), resistance training + synbiotics (n=15), and synbiotics only (n=15). The resistance training program consisted of 10 minutes of warm-up, resistance training at 40-65% of one-repetition maximum, and 10 minutes of cool-down, performed three times a week for 12 weeks. The resistance training + synbiotics and synbiotics groups also consumed one capsule daily one hour after lunch. Biochemical indices were measured at the end of the intervention. Data were analyzed using SPSS version 26 with paired t-test, one-way ANOVA, and Bonferroni post hoc test. The ankle brachial index (ABI) significantly increased in the resistance training + placebo(p=0/002) and resistance training + synbiotics groups(p=0/017). The cardio-ankle vascular index (CAVI) resistance training + placebo(p=0/005) and resistance training + synbiotics groups(p=0/002), LDL levels resistance training + placebo(p=0/018) and resistance training + synbiotics groups(p=0/013), triglycerides resistance training + placebo(p=0/049) and resistance training + synbiotics groups(p=0/031), body mass index (BMI), body fat percentage resistance training + placebo(p=0/0001) and resistance training + synbiotics groups(p=0/0001), were significantly decreased the resistance training + placebo and resistance training + synbiotics groups, glycated hemoglobin (HbA1c) levels in all three groups(p=0/001), and fasting glucose levels in resistance training + synbiotics group(p=0/019)were signifinicantly decreased.Also nitricoxide levels does not show any significant change in any goups. Additionally, muscle strength of the back, hamstrings, and triceps brachii was increased in the resistance training + placebo(p=0/001) and resistance training + synbiotics groups(p=0/003). The concurrent use of synbiotics and resistance training may be effective in reducing arterial stiffness, HbA1c, and lipid profile in postmenopausal women with type 2 diabetes