Abstarct: Due to the dependence of arterial stiffness responses on exercise and nutrition, the aim of this study was to investigate the relationship between arterial stiffness response after endurance exercise and glucose intake with basal vitamin D levels in inactive men. The present study was quasi-experimental and the statistical sample consisted of 45 inactive men in Shahroud with an age range of 20 to 45 years. All samples were present in the laboratory on an empty stomach and blood samples were taken to measure vitamin D. On the next day, body composition analysis, arterial stiffness and blood sugar were measured on an empty stomach, then 30 minutes of endurance activity with 65% of maximum heart rate. The heart was on a treadmill, after which it rested for 30 minutes, after which blood glucose was measured by a glucometer. Then 75 g of glucose was consumed and then rested for 30 minutes and arterial stiffness and blood sugar were measured again. Data were analyzed using SPSS software version 21 and analysis of variance with repeated measures and Pearson correlation at a significant level (P ≤0.05). The results showed that changes in CAVI response following exercise and glucose consumption were significant with baxseline vitamin D levels in inactive men (P ≤ 0.032). Changes in ABI response following exercise and glucose uptake with baxseline vitamin D levels were not significant in inactive men (P≤ 0.294). There is a significant indirect relationship between basal CAVI and basal vitamin D levels in inactive men (P≤ 0.009). There was no significant relationship between baxseline ABI and baxseline levels of vitamin D in inactive men (P≤ 0.098). Due to the results and research, there is very little need for these findings to be reviewed and confirmed in future research, but it seems that basal levels of vitamin D in the vascular response to exercise and glucose consumption can be effective.