Abstarct: The purpose of this study was to investigate the effect of garlic allicin supplementation on delayed onset muscle soreness and cell damage indices after an exhausted of the exercise session. 30 non-athletes participated in this study and randomly divided into 2 groups that included: placebo group (resistance + placebo) and supplementation group (allicin + resistance training supplement). The intensity of the exercise program was done with an 85% in each set. 14 days before the starting of the exercise program, all of the groups consumed the placebo and allicin supplementation (70 mg of allicin and placebo) each day. Lactate dehydrogenase and creatine kinase variables also were measured before, one hour after resistance training, 24 hours, 48 and 72 hours after resistance training. The two-way ANOVA test was used. The results showed that the use of allicin supplementation does not have a significant effect on the creatine kinase compared to the placebo group but significant effect on lactate dehydrogenase. Therefore, it is recommended to use long-term supplementation of allicin.