Abstarct: Bacground: Oxidative stress is caused by the imbalance between the production of free radicals and active oxygen species one hand and antioxidant defense on the other. When performing intense endurance exercises (aerobic), the production of activated oxygen species increases, and it is thought that the main source of these materials is mitochondria of active muscle cells. With increasing intensity of physical training, especially severe aerobic exercises, oxidative stress, lipid hyperoxidation and insufficient antioxidant defense system. Therefore, among athletes, the use of antioxidant supplements has become widespread. In this study, we investigated the antioxidant effects of taurine supplementation in two ways. Methodology: The present study was a semi-experimental pre-test and post-test. The statistical population consisted of basketball and volleyball boys in Shahrood city. Of these, 20 qualified subjects were selected and divided into two groups: complement (n = 10) and placebo (n = 10). The first method of administration was 2 hours before the exhaustive exercise activity.Subjects of complement group received 1,000 mg of taurine, 2 hours before the test. Also, the same amount of starch was given to the placebo group. In the second method, subjects received 14.5 grams of taurine daily for 14 days, and after 14 days they were tested. The test of the exhausting sport activity was modified Bruce Test. In the first method, blood samples were taken from the subjects in two phases, and the subjects were fast at first load. In the second method, samples were taken three times (before loading, after 14 days before the test, after the test). Then, after completing all stages, plasmas of the blood sampling stages, were sent to the laboratory to analyze the data. Results: The findings showed that consumption of Taurine 2 hours before activity and its short duration download did not have a significant effect on serum levels of superoxide dismutase and catalase enzymes. In addition, Taurine consumption with two methods did not differ significantly between the two groups on the time to get exhausted in the supplement and placebo groups. Of course, in the first method, there was a difference between the two groups, but this difference was not high.