Abstarct: Introduction. Increasing inactive behaviors is one of the growing health problems in different societies that secretly increases the risk of chronic illnesses, including non-alcoholic fatty liver in individuals. The aim of this study was to investigate the effect of eight weeks’ aerobic exercise and jujube extracts on non-alcoholic fatty liver in middle-aged women. Methodology. The present study was semi-experimental and research subjects of 40 middle-aged women workers aged 25 to 55 years old from Golestan universities and Gorgan Agricultural and Natural Resources Sciences. The participants were selected using ultrasonography and blood test for diagnosed fatty liver by available sampling method. They were randomly assigned into four groups: control, aerobic training, juvenile extract, aerobic training with jujube extraction They were Fatty liver measurements were performed using sonography and blood tests. Patients with confirmed liver fat in ultrasonography had a 1.5-fold increase in serum ALT and a ratio of AST to ALT to less than one; they were known as exits to patients with fatty liver. The program of the exercise groups was performed for 8 weeks, with a frequency of 3 sessions per week and each session for 35 to 50 minutes. Data were analyzed by SPSS software version 22 and t-test and independent t-test at a significance level of 0.05. Findings. The results of this study showed that eight weeks aerobic training, jujube extracts and a combination of aerobic training and jujube extracts had a significant effect on the level of ALT and AST liver enzymes (p≥0.05). However, there was no significant difference between the eight weeks of aerobic training and the use of jujube extract on liver enzymes in middle aged women (p≤0.05). Discussion and Conclusion. Considering that the ALT and AST liver enzymes have been affected by aerobic exercise, the use of jujube extracts and a combination of both, it is recommended to use exercise and exercise jujube extracts to reduce the amount of fat in the liver. To further confirm the results of the research and increase the external validity of the research, future researchers will be advised to conduct a similar study in more samples and in other communities.