Abstarct: Taurine seems to be effective in reducing muscle damage due to its cellular protective features and cellular function against the oscillation of the outside environment. So the purpose of this study was to investigate the effect of 2 weeks of supplementation of taurine on the response of muscle damage indices to an exhaustive activity session in physical education students. Methodology: In a semi-experimental study, 22 female physical education students (age: 22.43 ± 1.70 years, weight: 58.92 ± 13.81 kg, height: 163.75 ± 5.10 cm, mass index Body: 219.9 ± 4.70 kg / m2) were randomly assigned to placebo (12 subjects) and supplemental (10 subjects). Bruce's test was used as an exhaustive test. During the two weeks of loading, the subjects consumed 15 mg / kg of body weight each day to supplement the taurine or placebo, which is contained within 3 capsules of 500 mg. In the pre-test and post-test stages, blood samples were taken before and after the exhaustive activity to determine serum creatine kinase and lactate dehydrogenase levels. Data were analyzed using SPSS software version 16. The normal distribution of data was assessed using Shapiro-Wilk test and the combined analysis of variance (4 × 2) and dependent t-test was used for data analysis at a significant level of P <0.05. Results: Data analysis showed that Taurine supplementation had no significant effect on the response of creatine kinase and lactate dehydrogenase levels (P <0.05). Also, it was observed that the exhaustive activity did not significantly increase the levels of creatine kinase and lactate dehydrogenase in both pretest and post test (P <0.05). Conclusion: According to the results of this study, the addition of taurine did not have a significant effect on the responses of creatine kinase and lactate dehydrogenase levels, as well as exhaustive activity had no significant effect on these levels. but due to the research constraints, further research is needed.