Abstarct: The human body is a complex network of organs and tissues with distinct function. Various factors, such as aging, occur in the body, which can lead to damage to tissues or disrupt their normal function. One of the most important of these tissue is bone. Regarding the composite structure of the natural bone, by designing polymer-ceramic composite materials, an ideal scaffold for bone tissue engineering can be obtained to enhance mechanical, biological and physical properties. The selection of biodegradable polymer-ceramic nanobiotic composite for tissue engineering applications has an important role in regulating the cells due to its nano-bio structure. Scaffolds usually have a porous structure similar to extracellular matrix, acting as a frxamework and support for cells, so that the cells can attach to them, grow and multiply and differentiate, and ultimately secrete the extracellular matrix. Scaffolding also acts as a carrier for cells, growth factors and other biomolecules. A scaffold must be able to imitate the structure and properties of the human tissue to control and guide the process of creating tissue. Tissue engineering science is a combination of molecular biology and material engineering. Fast degradation and good biological activity these nanobiotic composite make them a suitable material for medical applications. The purpose of this study is to make the hydroxyapatite / hyaluronic acid / polyvinyl alcohol nanobiotic composite as a bone filler. Nanobiotic composite were prepared by casting method. FTIR test, impact strength, SEM and XRD were obtained from samples and their results were investigated. FTIR results do not indicated the formation of a new chemical composition. The compressive strength indicated that the sample containing hydroxyapatite / hyaluronic acid / polyvinyl alcohol / gelatin had the highest numerical value. Microstructural analysis of SEM the formation of scaffolds for the sample, including hydroxyapatite / hyaluronic acid/ polyvinyl alcohol / gelatin, shows that the porosity, porosity size and bonding of these samples indicate that the sample is suitable for tissue engineering applications. The XRD analysis determines the amorphous nature of the sample and, given the similarity of the natural bone XRD diagram, this composites can be used as a bone filler.