Abstarct: Austenitic stainless steels are the most widely group of engineering alloys used in many fields such as aerospace, chemical, and oil and gas industries. The problem with austenitic stainless steels is their poor tribological properties and their low corrosion resistance in some environments. Surface coating is one of the common ways to improve the mentioned poor tribological properties. The purpose of this study is to investigate the effect of substrate nanostructure on the mechanical, corrosion, and tribological properties of TiN coating applied on 301 AISI stainless steel. For this purpose, TiN laxyer was deposited on nanostructure and coarse grain substrate using cathodic arc evaporation method. X-ray diffraction (GIXRD) was used to identify the phases in the coating. The microstructure and thickness of the coated specimens were investigated by scanning electron microscopy (FESEM). To investigate topography and surface roughness, AFM analysis was performed on coating surfaces. Corrosion resistance was investigated by corrosion tests (electrochemical potentiodynamic polarization and impedance). The wear resistance and friction coefficient of the coating were evaluated by pin-disk test. The results of FESEM and XRD revealed that under the same conditions of coating application, the coating thickness on the ultrafine sample was higher. The corrosion resistance was improved by applying TiN coating on the samples, and the corrosion resistance of the ultrafine sample is much better than that of the coarse grain. The wear properties of the specimens were improved by coating. The fine-grained TiN coating exhibited less thickness change than the coarse grain specimen after two hundred meters.