Abstarct: At this study, the adsorption of organic pollutant as methylene blue and Ni(II) ion with nano adsorbents such as Pythium sp. and Athelia bombacina fungus has been studied. The results of the SEM analysis indicated that the size of adsorbent is in the nano scale. Also, the results of XRF, show that the main constituents of Pythium sp. is P2O5, CuO and ZnO and the main constituents of Athelia bombacina is P2O5, K2O and CaO. The analysis of FT-IR indicated that the OH– functional group is the major functional group of pollutant adsorption. CHNS analysis indicate that carbon dosage 43.11% and 40.15 for Pythium sp. And Athelia bombacina, respectively. According to the obtained results, it was obvious that the pH optimal = 10, dye concentration = 10 ppm and the nanoabsorbent dosage = 0.005 so, maximum removal of dye by Pythium sp. and Athelia bombacina is 77.58% and 75.98%, respectivly. Kinetic and equilibrium studies show that the process of adsorption on both nonoabsorbent is fitted with pseudo-second-order model and Langmuir isotherm. Thermodynamic studies show that the sorption of dye by Pythium sp. and Athelia bombacina are exothermic/spontaneous and endothermic/spontaneous, respectivly. Then, the sorption of Ni(II) ion under the listed conditions and in the batch system on Pythium sp. and Athelia bombacina were studied. It was found that the maximum adsorption by Pythium sp. and Athelia bombacina at the optimal pH=6.5, mextal concentration = 10 ppm and the adsorbent dosage = 0.015 g are 66.2 and 71.5%, respectivly. The adsorption of Ni(II) ion on nanoabsorbent follows the Langmuir isotherm and pseudo-second-order kinetic. The process of adsorption on both nanoadsorbents are exothermic and spontaneous.