Abstarct: This study consist of the application trichoderma harzianum and actinomucor elegans var. elegans fungus as nano-adsorbents, for removal of organic pollutant (dye) and inorganic pollutant (toxic element). Experimental consist of SEM, XRD, CHNS and FT-IR were used to identify the chemical and physical properties of adsorbents. The results of the SEM analysis indicated that the size of adsorbent particles is in the range of nano. Also, the results of XRF, show that the maximum amount of trichoderma and actinomucor were Silicon Dioxide and Potassium Oxide and Phosphorus Pentoxide and Calcium Oxide, respectively. The analysis of FT-IR indicated that the functional group –OH as the main functional group of pollutant adsorbing process. The removal of malachite green dye and Cu(II) ion were studied. To investigate the adsorption process, several parameter consist of pH, adsorbent dosage, concentration and temperature were studied. The results show that removal rate of dye is more than 90%. also, the adsorption process were studied in equaliberum and kinetically and thermodynamically. According to isotherm models, the adsorption process of malachite green on both fungus follow up Freundlich isotherm model. Kinetic studies show that justify both the adsorption process by the pseudo-second order kinetic model. The thermodynamic process of adsorption of malachite green by trichoderma harzianum and actinomucor elegans var. elegans spontaneous and exothermic. It is bviusly that the maximum adsorption of Trichoderma nano-adsorbent, accured at the optimal pH 5.5, dye concentration (10 ppm), and the amount of nano-adsorbent 0.01 grams was 76.9%, and the maximum absorption of actinomucor nano-adsorbent, 59.8 percent. According to the isotherm models, the adsorption process of Cu(II) on both fungus follow up Tempkin model. Kinetic studies show that justify both the adsorption process by the pseudo-second order kinetic model. The thermodynamic process of adsorption of Cu(II) by trichoderma harzianum and actinomucor elegans var. elegans spontaneous and exothermic.