Abstarct: Bioleaching is a method for extracting copper from sulphide ores in which mextal recovery is done by bacteria. Currently, a number of catalytic materials are used to improve the speed of the reaction to recover mextal sulfides. Among the catalysts, silver ion has the highest efficiency in accelerating the reaction. However, due to the high price of silver compared to copper, enough economic studies should be done to be able to use it as a catalyst in bioleaching. In this research, in order to recover copper from sulfide minerals, bioleaching experiments were conducted using moderate thermophilic bacteria and silver catalyst. Shaking flask bioleaching experiments were conducted in two stages using two types of samples: sulfide concentrate sample and sulfide low-grade soil sample. The first stage tests were performed without designing the experiments for samples with a size of 75 microns for both sulfide concentrate and low grade soil. In the sulfide concentrate sample, with the consumption of 30 ppm of silver sulfate (Ag2SO4), the maximum recovery of 50.46% was obtained. Also, in the sample of low-grade sulfide soil, when the consumption of silver sulfate was equal to 5 ppm, the recovery of 91.70% was obtained. However, the second stage tests were conducted with the design of experiments using DX11 software only for the sulfide concentrate sample, and due to the high costs of silver ion for the low-grade soil sample, the design of the experiments was not performed. The second series of experiments were conducted by examining two parameters: silver ion dosage and particle size with a full factorial design. The results indicated that with the consumption of 750 ppm of silver sulfate and the particle size of 44 microns, the maximum recovery is 72.60%. Also, in order to expand the test conditions, first a bioleaching test was performed for a 75-micron sulfide concentrate sample in a one-liter cylindrical glass reactor. Copper recovery in this test was 38.05%. Then, two column bioleaching experiments were performed for a low-grade sulfide soil sample in one-meter columns with two different concentrations of silver ions (zero and 5 ppm). For the control sample (without silver ions), the recovery was 39.58% and for the concentration of 5 ppm, the recovery was 52.82%. On the other hand, in order to recover silver, the bioleaching residue of sulfide concentrate sample was subjected to leaching operation by thiourea, and the silver recovery was 52.5%. The results of the economic analysis indicated that the use of silver sulfate for low-grade soil samples is economically viable. ide soil; Silver ion; Economic studies.