Abstarct: In experimental prespective, using microfluidic chips are new methods of cellular evaluation. In recent years due to their different functions such as: chemical analysis, biomedical, chemistry, eco-system, food industry, water calibration experimentals, new methods called trapping particles and cells, liquid direction in micro and nano sections had been emphasized. Lots of microfluidic methods in particles separation, using direction course or generated forces by electricity, optic, magnetic, hydrodynamic or gravity had been developed. In this study, we tried to invedtigate the concepts of the dielectropharesis force and its role in how to trap microparticles. Dielectropharesis force is created when dielectric particles are exposed under non- uniform electric field. In the following method, there is no need for charged particles but is dependent on polarized paeticles. The effectivity of the force relies on electrical properties of particles and the environment. In this dissertation, primarily the original model which is practically enabled to be made is simulated by COMSOL software to be able to analyze direction of dielectric particles that existed in fluid flow to the specific reservoir which is installed for trapping and accumulation of the particles. Partcles are influenced by non-uniform electric field after entering the main channel. This microchannel has six electrodes. Two electrods are exposed on the bottom of the microchannel that are directly connected to the voltage resource. One of these two electrodes is exposed on the beginning of the microchannel that due to the electric field generated causes repelling force on particles. The other electrode is exposed inside the chamber that causes generation of absorbing force on particles. Other four electrods are exposed on top of the microchannel that work as ground electrod. The factor that is called clausiuse mossoti factor, is effective for dielectropharesis force generation. This factor depends on the electrical properties(diectric constant, electrical conductivity) of particle and fluid and frequency that connected to voltage resource. By changing the input frequency, this factor takes on positive or negative value and consequently positive or negative dielectropharesis force is generated. Influenced particles by this forces are absorbed or repelled. So by this method, we can creat definite path for particles movement and expositionary in a specific place. In this study, microparticles(saccharomyces cerevisiae) and a liquid of ,methanol, methylene blue and dionized water are selected as practical liquid.fluid flow containing suspended particles, are directed to the channel by speed of 250 micrometer per second. This flow primarily is influenced by electric field and applied frequency on electrode ( 46 V and 2.1 MHz) with electrods that existed at the beginning of the channel(focusing electrods). then, the flow is influenced by electric field and applied frequency on electrode (50 V and 1 KHz) that existed inside chamber and the particles are directed to the inside chamber and accumulated there. In this study, in numerical analysis, yeast cells with two different diameters (5,8micrometer) are separated from each other inside separate chambers by dielectropharesis force. Particles separation inside separate chambers can be regarded as great improvement in medical science. Changing in number and geometry of the electrodes can be influential on the effectivity of dielectropharesis forces on particles for being trapped inside the chamber. In this study, by innovation created on focusing electrod’s geometry, following results are achived. By changing in this geometry, more particles are directed to the center of the channel. Particles are focused by negative dielectropharesis force. They are focused not only on the top r bottom of the microchannel but it is also taken apart from the walls. This microchannel is practically has been experimentally tested. Contoroller parameters sach as: frequency, voltage and input speed are adjusted in a way that the fluid flow containing yeast cells with input speed of 250 micrometer per second is effected by electric field on attractive electrode (50 V and 1 KHz), particles are absorbed inside the chamber. Particle’s absorbtion inside the chamber deponds on their concentrate in focusing electrod zone.