Abstarct: One of the recent studies in fluid mechanics science is the microfluidics transportation analysis in micropumps. So far, various methods have been developed to increase the efficiency of AC electro-osmotic pumps with raised steps on the electrodes. In order to achieve a desired flowrate, it is crucial to optimize various electrode parameters such as dimensions, position, input voltage, etc. To date, a comprehensive systematic optimization frxamework, capable of accurately predicting an efficient electrode geometry is not available. Therefore, developing methods which can overcome these problems in micropumps are vital. In present study, in order to fabricate ACEO micropumps, the optimization of geometrical parameters of 3D electrode, such as width and steps height of each electrode and their displacement on the baxse electrodes in one pair (symmetric or asymmetric electrode), the electrodes gap, and also electrical characteristics including voltage and frequency have been investigated. The governing equations of flow comprising of electric domain and fluid domain have been solved using finite element method to investigate the effect of electrode geometry on slip velocity, which affects pressure and velocity of the fluid. Finally, this chip is fabricated by deposition of Pt electrodes on glassy substrate in order to measure the pumping velocities of dilute electrolyte KCl solution. Numerical simulation predicts an improvement in pump performance with increasing step height and distinguishing its displacement, at a given voltage(2.5V) and frequency(1kHz), which qualitatively matches the trend observed in experiment. Our results indicate that an optimal design results in a pump with the width (50µm) and steps height (5µm) of each electrode and their displacement (30µm) is capable of generating a high velocity, flow rate and pressure around 1.77mm⁄(s,) 14.9(µL)⁄(min )and 74.6Pa, respectively.