Abstarct: In recent decades, many of the weaknesses associated with concrete brittleness have improved with the development of fiber-reinforced concrete. This is due in particular to several developments involving the matrix, the fiber, the fiber-matrix interface, the composite production process, a better understanding of the basic mechanisms controlling their particular behavior, and a continually improving cost-performance ratio. Today, fiber-reinforced concrete has a high energy absorption capacity, which intensifies the interest in studying their behavior at loading rates above the static range. This study aims to investigate the effect of loading rate on the fibers-matrix interface behavior and its relationship with the bending behavior of fiber-reinforced concrete. For this purpose, the pull-out performance of three hooked fibers with different aspect ratios was evaluated by employing an innovative arrangement under various loading rates ranging from the static to the seismic level. Also, the bending performance of reinforced beams with one percent by volume of fibers according to (ASTM C1609) subjected to loading in the same strain rate range with pull-out tests. The digital image correlation (DIC) method was used to measure mid-span deflection and observe the pattern of crack propagation in the two-dimensional strain field. Experimental results reveal that tensile stress of fibers, the fiber-matrix bond strength, and the work done was increased with increasing loading rate. The bond strength per unit area for fibers with an aspect ratio of 92 is more than twice that of fibers with an aspect ratio of 50 at all loading rates. On the other hand, load capacity, toughness, and equivalent bending strength were calculated to evaluate the bending behavior with changes in loading rate. As the strain rate increases, the bending capacity 26% increases. But the strain rate sensitivity of toughness and equivalent flexural strength to changes in strain rate decreased with an increasing deflection in the middle of the span. baxsed on the composite theory, the fiber-reinforced concrete bending strength under dynamic loading can be effectively predicted by using the fiber-matrix bond, the dynamic increase coefficient and, the fiber-related parameters.