Abstarct: The aim of this study was to use micro-nano bubble (MNB) technology to separate oil and iron from aqueous solution in a batch system. In this study, several steps including flotation with MNB, pack coagulant and filter paper were taken to separate the oil in the emulsion while ozone MNB was used for oxidation of soluble iron. The influence of factors affecting the flotation process, pH size, bubble size, type of inlet gas and initial concentration for fat separation were investigated. Also, the properties of the precipitated iron oxides were determined by XRD, XRF and SEM analyzes. baxsed on the results, it was found that the maximum percentage of oil removal from the emulsion at a concentration of 430-440 mg / l by flotation with Minab technology, air stone and aeration with a compressor, with a fixed volume of 1.8×10-4 cubic meters of air, respectively 64.8, 57.2% and 55.5%. Oil removal at pHs of 3, 7, 9 and 10 were measured as 32.9, 45, 53.6 and 62.3%, respectively. According to the results of XRD, XRF and SEM, the oxidation of soluble iron with ozone led to the production of iron oxide nanoparticles. In general, the separation of fat and iron by flotation of Minab technology is more efficient than flotation with different bubble sizes.