Abstarct: Fatigue is one of the most important causes of damage in asphalt pavement. Four point beam fatigue test, wich is considerd as a destructive test, can be used to determine the fatigue properties of asphalt mix. The destructive tests will always have significant costs. On the other hand, destructive tests can cause damage to the desired structure, so it will not be possible to repeat the test. This study intends to minimize the need for destructive tests by investigating the correlation between two destructive tests inculuding four point beam fatigue test and three point bending test with Portable Falling Weight Deflectometer (PFWD) wich is a non-destructive test. For this purpose, in 25 stations, the data were took using PFWD and then at 7 stations road surface asphalt was cut and beam speciments with standard dimension were prepared and were subjected to fatigue and bending tests. The data are divided into two groups: One group of data and samples has taken from the wheel path and another from the outside of the wheel path. Fatigue tests were conducted in strain-controlled mode with a sinusoidal load applied with frequency of 10 Hz (without rest) at 25˚C and 0˚C. Fatigue life was analyzed by the stiffness reduction method. Subsequently, by analyzing the PFWD's data and the output of fatigue and bending tests, it is found that more displacement under impact load of PFWD occurs on the wheel paths due to fatigue and its cracks. Finally, linear relation and high correlation were achieved between results of PFWD and fatigue test. In a way that by increasing the displacement achieved by PFWD, fatigue life decreases. As well a model using linear regression is presenred for samples fatigue life in two different temperature, 25 ˚C and 0 ˚C.