S713 : The effect of seed pretreatment and foliar application of humic acid, green tea and coffee extract on growth, yield and physiological traits on cowpea (Vigna unguiculata L.)
Thesis > Central Library of Shahrood University > Agricultural Engineering > MSc > 2024
Authors:
Reza Sabaghian [Author], Mehdi Baradaran Firouzabadi[Supervisor], Mostafa Heidari[Supervisor], [Advisor]
Abstarct: Todays, there are several solutions to increase the resistance of plants against harsh environmental conditions. Optimum and balanced plant nutrition using organic compounds is one of the ways to increase the resistance of plants against harsh conditions. In order to investigate the effect of humic acid, green tea extract and coffee extract on agricultural and physiological characteristics of cowpea, an experiment was designed and implemented in 2023 in a laboratory and a farm in Semnan city. The laboratory part consisted of three separate experiments. In the first experiment, the experimental treatments included seed pretreatment with coffee extract at eight levels (zero, distilled water, 10, 20, 30, 40, 50 and 60% by volume), the second experiment included pretreatment with green tea extract at eight levels. (including zero, distilled water, 10, 20, 30, 40, 50 and 60 percent by volume) and the third test of pre-treatment with humic acid at eight levels (including zero, distilled water, 150, 300, 450, 600, 750 and 900 mg/liter) that all three experiments were carried out in the form of randomized complete block design in three replications in the germinator environment. The field part was implemented in the form of a factorial experiment baxsed on a randomized complete block design in three replications. The treatments in this section include seed pretreatment at five levels (control, distilled water, humic acid (750 mg/liter), green tea extract (10% concentration) and coffee extract (10% concentration) It was done for six hours. and foliar spraying at four levels. The levels was humic acid (750 mg/liter), green tea extract (10% concentration) and coffee extract (10% concentration). The results showed that in laboratory conditions, seed pretreatment with distilled water and 10% concentration of green tea extract increased the vigor index (seed root) by 4.75% and 7.75%, respectively, compared to the control. Seed germination in seeds pre-treated with 10% concentration of coffee extract was 6.85% more than the control Also, when the seeds were pretreated with 750 mg/liter of humic acid, the seed germination increased by 22.57% compared to the control. In the field, pretreatment of seeds with humic acid, green tea extract, coffee extract, and distilled water caused a significant increase in stem diameter, leaf dry weight, stem dry weight, and total dry weight compared to the control. Also, foliar spraying of humic acid, green tea extract and coffee extract significantly increased stem diameter, leaf dry weight, stem dry weight and total dry weight. In the physiological traits investigated in field conditions, seed pretreatment with the above treatments increased the relative water content of leaves, leaf surface index, chlorophyll a, chlorophyll b, carotenoid, membrane stability index and decreased malondialdehyde. Also, foliar spraying with humic acid, green tea extract and coffee extract also increased the above physiological traits and decreased malondialdehyde. Also, foliar spraying with humic acid, green tea extract and coffee extract also increased the above physiological traits and decreased malondialdehyde. In the study conducted in this research, seed pretreatment and foliar spraying with the above treatments reduced fructose, glucose, and carbohydrates, as well as increased sucrose. In the pretreatment of seeds with humic acid, compared to the control, the biggest decrease was 24.04% in fructose, 28.44% in glucose, 24.79% in leaf soluble carbohydrates and 19.62% increase in sucrose.In the pretreatment of seeds with humic acid, compared to the control, the biggest decrease was 24.04% in fructose, 28.44% in glucose, 24.79% in leaf soluble carbohydrates and 19.62% increase in sucrose. Also, foliar spraying with humic acid resulted in the greatest reduction of 12.18% in fructose, 16.30% in glucose, 10.24% in leaf soluble carbohydrates and an increase of 7.64% in sucrose compared to the control. In the study of seed yield characteristics, seed pretreatment and foliar spraying with the studied treatments showed a significant increase in the characteristics of number of seeds per pod, number of pods per plant, hundred seed weight and seed yield. In the study of the qualitative characteristic of seed protein percentage, pretreatment of seeds with humic acid, green tea extract, coffee extract and distilled water all increased the percentage of seed protein in a statistical group, and the highest increase was related to pretreatment with humic acid at the rate of 3.49%. was a witness. Finally, within the scope of the research, it is possible to pre-treat seeds with 750 mg per liter of humic acid, green tea extract with a concentration of 10% recommended to improve germination and increase seed vigor in cowpea. Seed pretreatment and foliar spraying of humic acid at the rate of 750 mg/liter and green tea at a concentration of 10% are recommended in the field.
Keywords:
#coffee extract #pretreatment #foliar spraying #green tea extract. Keeping place: Central Library of Shahrood University
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