Abstarct: Salinity is a worldwide environmental problem that negatively affects crop yield and thus poses a threat to global food security. According to increase the threat of salinity, understanding the mechanism of salt tolerance in plants and finding ways to develop salt-tolerant plants will take time. Aquaporin proteins (AQPs) include a group of intrinsic membrane proteins that are very important for plant survival under stress conditions, including salt stress. The intracellular movement of water in plants is facilitated by small inner membrane proteins (21-34 kDa) called aquaporins. The exxpression of aquaporin genes is different depending on the organ, hormone and abiotic stress treatment (drought, heat, salinity and cold). During the response of plants to adverse stresses, aquaporins play a prominent role in membrane water transport baxsed on received upstream signals. Aquaporins play an important role in the tolerance of different plant species to abiotic stress and also have various physical roles in dealing with biotic stress. Due to the importance of this gene family, studies have been conducted in the field of investigating the function and regulatory system of these genes, although many of their molecular aspects are still unknown. baxsed on this, the aim of this study is to carry out an extensive genomic investigation of the aquaporin gene family in durum wheat using bioinformatics tools and investigating the exxpression of some members of this gene family in response to salt stress. The results showed that there are 80 AQP genes in durum wheat, which are classified into four groups baxsed on phylogenetic analysis. baxsed on the analysis, a large number of duplications were observed between the members of the AQP gene family, and also a high diversity in response to post-translational modifications was observed between them. The exxpression pattern of AQP genes showed that these genes are mostly expressed in roots and have the highest exxpression increase in response to simultaneous heat and salt stress. Also, qPCR data showed that AQPs are more induced in delayed responses to salinity stress. The results of this research created a preliminary understanding of the process of evolution and function of the genes of the aquaporin family, which will be effective in future works related to the introduction of salt resistance mechanisms in durum wheat.