Abstarct: This study focuses on Ganoderma lucidum, a one-year mushroom with medicinal properties from the Ganodermataceae family. Known as Lingzhi in China, Rishi in Japan, and Yung Zahi in Korea, this mushroom is traditionally used for treating various ailments such as insomnia, shortness of breath, memory enhancement, kidney and liver diseases, arthritis, and asthma. It contains essential biologically active substances like sterols, proteins, polysaccharides, triterpenoids (ganoderic acid), polyphenols, and melanin. Nanoscale particles, characterized by their larger surface area, exhibit better solubility and surface performance compared to their larger counterparts. Hence, this research aims to explore the optimal pH and concentration of nanoparticles in the Ganoderma lucidum mushroom culture medium, specifically focusing on growth rate and the production of secondary mextabolites, including ganoderic acid and phenolic acid.The investigation involved yellow and red Ganoderma lucidum mushroom culture media containing nanoparticles of magnesium oxide (MgO), titanium dioxide (TiO2), iron oxide (Fe3O4), zinc oxide (ZnO), and silicon dioxide (SiO2). The results displayed that a pH of 5.5 generally yielded the best outcomes for growth and secondary mextabolite production. Further analysis revealed that iron oxide and magnesium oxide nanoparticles exhibited the highest growth rate and production of phenolic acid and ganoderic acid. Consequently, a subsequent experiment was conducted to assess the impact of different concentrations of these nanoparticles. It was observed that higher concentrations of iron oxide nanoparticles led to improved growth rates, while lower concentrations resulted in enhanced production rates of phenolic acid and ganoderic acid. Similar trends were observed for magnesium oxide nanoparticles. These findings highlight the significance of optimizing pH and nanoparticle concentrations in the Ganoderma lucidum mushroom culture medium to enhance its growth and secondary mextabolite production. Such insights contribute towards developing strategies for maximizing the therapeutic potential of this valuable medicinal mushroom.