Abstarct: The present study was designed to evaluate the impact of application of Rhizobium bacteria and mycorrhizal fungi as biological fertilizer and iron sulphate as a fertilizer on yield of chickpea and some soil characteristics. Factors were factorial arrangement of Mesorhizobium ciceri bacteria (inoculated and non-inoculated), mycorrhizal fungi (Glomus mosseae, Glomus intraradices and non-inoculated) and various amounts of iron sulphate fertilizer (zero, 40 and 80 kg per hectare), baxsed on randomized complete block design with three replications. The experiment was conducted in the city of Sabzevar in spring and summer of 1390. The results showed that application of Rhizobium increased grain yield (by 23.4%) and all yield components, except number of seeds per pod and number of pods per plant. The concentration of zinc and sulphur in seed and phosphorus in the soil was also affected significantly. Mycorrhizal symbiosis enhanced grain yield (by 34.54%), yield components, and concentration of zinc, iron, sulphur and phosphorus in the grain. For these cases, G. mosse was more effective than G. intraradices. Application of ferrous sulphate fertilizer increased yield, yield components, amount of sulphur, zinc and protein. Double interactions were significant on plant height, number of secondary branches, seed yield, percent of nitrogen and protein, phosphorus, sulphur, zinc, iron, phosphate and sulphate (available in the soil). Triple interactions appeared to be significant on number of secondary branches, the percentage of nitrogen, protein, phosphorus and concentration of zinc and iron, sulphate (available in the soil).