Abstarct: In this research , the fauna of prostigma mite and their biodiversity were investigated in the northern regions of Damghan city in 2020-2021. Soil sampling was collected from areas that have a high humidity. In this study, a total of 6952 mite were isolated and slides, and a total of 32 species from 15 families of preostigma mite were identified, among which three species are new to the fauna of the world, and two species were reported for the first time from Iran. The order is marked with (*) and (**) respectively. By using biodiversity indicators in the six sampling areas, the Bdellidae family has the highest frequency (19.77 percent) and the Camerobiidae family shoewd the lowest frequency (0.14 percent). Cunaxidae: Cunaxa gordeevae Sergeyenko, 2009; C. grobleri Den Heyer, 1979; Cunaxoides croceus (Koch, 1838); Armascirus hastus Shiba, 1986; Scirula sp.nov. *; Bdellidae: Bdella muscorum Ewing, 1909; Biscirus iranensis Paktinat-Saeej & Bagheri, 2015; Cyta latirostris (Hermann, 1804); C. coerulipes (Dugès, 1834); C. kreiteri Barbar & Ueckermann, 2017; Odontoscirus meridionalis (Thor, 1931); O. mazandaranensis Paktinat-Saeij, Bagheri & Hernandes, 2016; Spinibdella cronini (Baker & Balock, 1944); Cryptognathidae: Cryptognathus lagena Kramer, 1879; Favognathus distortus Kuznetsov, 1974; Favognathus mirazii Khanjani & Ueckermann, 2008; Raphignathidae: Raphignathus gracilis (Rack, 1962); Stigmaeidae: Eustigmaeus segnis Koch, 1836; Eustigmaeus caspianensis Bagheri and Paktinat Saeej, 2014; Trombidiidae: Allothrombium meridionale Berlese, 1910; Erythraeidae: Abrolophus sp. nov. *; Leptus trimaculatus(Rossi, 1794); Scutacaridae: Scutacarus echidna Berlese, 1905** ; Imparipes dilatatus Mahunka et Zaki, 1982. **; Camerobiidae: Neophyllobius asalii Khanjani & Ueckermann, 2006; Eupodidae: Eupodes clavifrons R. Canestrini, 1886; Cocceupodes paradoxus Weiss-Fogh, 1948; Eupodes hawaiiensis Strandtmann & Goff.**; Smarididae : Hirstiosoma latreillei (Grandjean, 1947); Cocceupodidae: Linopodes ambustus C.L.Koch, 1838; Microtrombidiidae: Enemothrombium sp. nov. *; Eutrombidium fathipouri Karimi Irvanlou, Kamali & Talebi, 2000; Rhagidiidae: Coccorhagidta Thor,1394; Calyptostomatidae: Calyptostoma gorganica Saboori & Soukhtsaraii,2012; All collected species are kept as permanent microscopic slides in the Museum of zoology or Professor Jalal Afshar Museum of Zoology, Department of Plant Medicine, University of Tehran (Karaj) Agriculture and Natural Resources Campus.