Abstarct: Sulfur is the fourth needed element for plants, especially for rapseed, and the deficiency of it in the agricultural soil of the world is increasing Sulfur an important part of metionin and cycteine amino acids, and has role in the formation of cholorophyll, enzyme activating compounds, vitamin B synthesis and photosynthesis. So an experiment has been done to study the effects of different sources of sulfur on growth, yield and physiological traits in brassicanapus at Shahrood University of Technology in marth 2019. Experimental treatments included the soil use of sulfur in 2 levels (consumption and lack of consumption), 3 levels of foliar application of sulfur sources (zero, liquid sulfur and sodium hydrosulfide) and 3 levels of seed pre-treatment with sulfur vitamins (zero, tiamin and biotin) as a factorial baxsed on randomized complete block with 3 replications. Use of sulfur solid was performed before planting and foliar application befor flowering. The results showed pre-treatment by thiamin increased number of pod in the bush and consumption also improved number of seed pre pod, oil percentage, plasma memberane stability and carotenoid. Sodium hydrosulfide in dis pre-treatment condition had positive effects on percentage and yield of protein. dry weight of leaf and pod, grain protein and rwc was increased by foliar application of liquid sulfur. Also interaction of sodium hydrosulfide and pre-treatment of tiamin increased pod dry weight, number of pod in the bush, chlorophyll a and b, oil percentage and protein yield. The percentage and yield of protein was increased by the usage of sulfur soil. Interaction of liquid sulfur and sodim hydrosulfide on biotin increased many physiological and qualitative traits. Generally in accomplished experiment, sulfur soil consumption and tiamin pre-treatment were more effective on considered traits, alone and in the combination whit other treatments too. Also the interaction of foliar application of sodium hydrosulfide and tiamin pre-treatment led to improve the most of agricultural, physiological and qualitative traits of brassica.