Abstarct: Intensive agriculture with high consumption of chemical fertilizers, especially nitrogen, guarantees high yields, but in this regard, it increases costs and environmental pollution. One of the main goals in sustainable agriculture is the use of biofertilizers in the ecosystem of crops with the aim of eliminating or significantly reducing the consumption of chemical inputs. For this purpose, to evaluate the effect of Barvar1 biofertilizer along with the combined application of spent mushroom compost (SMC) and nitrogen fertilizer on the agronomic characteristics of chickpea (Cicer aretinum), a factorial experiment in a randomized complete block design with 4 replications and 48 treatments in year 1397-1398 was performed in Firoozeh city. Experimental treatments was included biological fertilizer used in Barvar1 biofertilizer which contains bacteria of Azotobacter vinelandii strain O4 in two levels of bacteria (consumption and non-consumption) and combined application of SMC and nitrogen fertilizer including 6 levels of (consumption 100 kg urea fertilizer per hectare, consumption of 3 tons of SMC per hectare, combined consumption of 50 kg of urea + 1.5 tons of SMC per hectare, consumption of 200 kg of urea fertilizer per hectare, consumption of 6 tons of SMC per hectare, combined consumption of 100 kg of urea + 3 tons of SMC Per hectare). The first irrigation was performed immediately after planting and the subsequent irrigations were performed every 7 days in a drip manner with the same type tape in all treatments. The results showed that the number of pods with using 6 tons of compost and both levels of biological fertilizer, soil available phosphorus with application of 100 kg of urea and application of biological fertilizer, soil urease enzyme with use of 200 and 100 kg of urea and biological fertilizer, the number of main branches with use of 100 kg of urea and 6 tons of compost and non-use of biological fertilizer, chlorophyll b with use of 1.5 tons of SMC and 50 kg of urea and 200 kg of urea consumption of biofertilizer , carotenoids with 1.5 tons of SMC and 50 kg of urea increased significantly. Also, the interaction effect of biological fertilizer and SMC on grain yield, anthocyanin, biological yield, soil organic carbon, number of seeds and number of pods was significant. Therefore, SMC and biological fertilizer of Azotobacter can be considered as a suitable alternative to chemical fertilizers for sustainable agriculture.