Abstarct: Tomato, as a climatic fruit, is a perishable vegetable that has a low shelf life in the post-harvest stage at room temperature. This study was conducted to investigate the effect of 1-MCP and titanium oxide nanocomposite film packaging on physicochemical properties of two cultivars of yellow cherry (reinhards goldkirsche) and red (blackcherry) cultivars in the postharvest stage under cold storage. The results of this experiment showed that over time, the post-harvest quality of fruits in both cultivars decreased so that the lowest percentage of weight loss, the most changes in tissue firmness, pH, soluble solids content and titratable acidity in fruit packaged with polyethylene films were observed. Also, the highest contents of chlorophyll, carotenoids, anthocyanin and non-reducing sugars were obtained in fruits treated with 1-MCP and packed with nanocomposite films. On the other hand, components related to appearance color such as brightness index, chroma, hue and total color changes under the effect of 1-MCP treatment and titanium oxide nanocomposite films had less changes during the storage period. The lowest ascorbic acid, free radical scavenging capacity and antioxidant enzyme activity were obtained in both cherry tomato cultivars in fruits without 1-MCP treatment and packed with polyethylene films. 1-MCP treatment and titanium oxide nanocomposite films were more effective in controlling the rate of perishability than other treatments. It seems that the simultaneous use of 1-MCP as an inhibitor of ethylene synthesis and fruit packaging with titanium oxide nanocomposite films as active films in controlling ethylene production and microbial spoilage was able to improve the post-harvest quality of packaged fruits. Therefore, these treatments can be used as an industrial method to increase the shelf life of cherry tomatoes.