Abstarct: Iran is one of the major countries producing grapes that grows varieties and genotypes of various grape varieties..The aim of this study was to evaluate the genetic and morphological diversity of some genotypes in the grape collections in Shahroud Agriculture Research Station by using of morphological and SSR markers. In this study, 26 morphological traits were evaluated in 55 grape genotypes and used in molecular segmentation of 8 SSR primer pairs to evaluate 22 selected grape genotypes. The results obtained from the studied traits showed that there is a high variation in the traits being evaluated. The highest diversity index is related to Anthocyanin, petiole length, leaf shape, cluster density traits and the lowest diversity index is related to leaf angle. The results of correlation coefficients showed positive and significant correlation between some traits such as leaf angle with vein length, cubic width with cubic length, panicle width with panicle length and fruit color with petiole anthocyanin. The dendrogram of morphological traits was divided into four distinct groups at a distance of 15 to 10 cultivars and Dishui cultivar was grouped alone because of high anthocyanin. All SSR markers were polymorphic. The pair of primers used in this study showed binary and single bands in 22 genotypes. The cluster analysis of molecular data was performed baxsed on the UPGMA method and Jaccard similarity coefficient. In cluster analysis with SSR markers, cultivars were categorized and divided into four groups.The results showed that Cardinal cultivar is more distant than other cultivars and Askari Isfahan cultivar was grouped alone. The results of this study indicate the presence of molecular and morphological diversity among Shahrood collection grape cultivars.