Abstarct: The aim of this experiment, mycorrhizal symbiosis, thiobacillus bacteria and sulfur fertilizer effects on growth and yield of corn were studied. The experimental design was factorial in the baxse on randomized complete blocks design with three replications. Treatments consisted of mycorrhiza inoculation, in two levels inoculated (M1) and non-inoculated (M0), thiobacillus, in two levels inoculation (T1) and control (T0) and three levels of sulfur (S0: 0, S1: 250 and S2: 500 kg.ha-1). Results show that inoculation of mycorrhiza, thiobacillus, and sulfur application decreased the pH and increased grain yield, grain number per ear, stem height, weight of 100 grains (except mycorrhiza), biological yield, leaf chlorophyll, and relative water content (except sulfur) significantly compared to controls. Also the investigate factors improvement grain nutriants content and oil of corn. Nitrogen content was onley effect by the using of sulfur, and thiobacillus incearsed the grain S of corn significantly compared to control. The thiobacillus application increased grain S content by 12.2%. Oil percentage of grain corn was significantly affected by thiobacillus and sulfur application. The interaction of sulfur and thiobacillus was, significant for all the traits studied except for biological yield. The interaction of mycorrhiza and thiobacillus on decreasing soil pH and increase on stem height were significant. Thiobacillus and sulfur application decreased of root colonization percentage. Also, interaction of mycorrhiza with sulfur and thiobacillus with sulfur on grain S and Fe content was significant