S484 : study of exxpression pattern of cellulose biosynthesis genes in cold-sensetive and tolerant tomato genotypes under low temperture
Thesis > Central Library of Shahrood University > Agricultural Engineering > MSc > 2018
Authors:
Abstarct: Tomatoes are tropical plants that are sensitive to low temperatures, and the reduction in temperature at all stages of growth leads to the destruction of the cell wall and thus the loss of cold resistance, which causes damage to the plant. In this research, the pattern of exxpression of cellulose biosynthesis genes and the content of certain enzymes under low-temperature stress in two cold-resistant (S. lycopersicum cv. Moneymaker) and cold resistant tomato (S.habrochaites LA1777) tomatoes were investigated. The results showed that CesA1, CesA2 and CesA5 genes had different exxpression patterns in the studied species, so that in S. lycopersicum cv. Moneymaker increased exxpression and in S. habrochaites LA1777 decreased exxpression. The CesA3 gene is one of the key genes in cellulose synthesis in the cell wall, whose destruction or destruction causes plant destruction and death, was induced in resistant species more than susceptible. The activity of the enzyme guaiac peroxidase increased as a result of cold stress, so that in S. habrochaites LA1777 more than S. lycopersicum cv. Moneymaker Also, catalase activity decreased under cold stress, which was higher in susceptible species than in S. habrochaites LA1777. The activity of malondialdehyde and proline increased, and the membrane stability in both species decreased, which decreased in S. lycopersicum cv. Moneymaker was more likely to be thought that the cold stress caused the destruction and destruction of the cell wall in the sensitive species S. lycopersicum cv. Moneymaker and increase the sensitivity to cold in this species.
The results of this study indicate the role of cellulose synthase genes in low temperature stress and as the first report on the pattern of exxpression of these genes, which can be used in genes functional studies.
Keywords:
#Tomato #CesA #Gayacol poroxidas #Catalas #Molondead #Membrane stability #prolin
Keeping place: Central Library of Shahrood University
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Keeping place: Central Library of Shahrood University
Visitor: