Abstarct: Mentha aquatica of the Lamiaceae family is one of the most important species of aromatic plants known for its medicinal properties. The therapeutic effects of this plant are for essential oils and poly phenols. The main phenolic compounds in tannins are phenolic acids, especially Rosemaric Acid and produces as a secondary mextabolite in these plants. To achieve secondary mextabolites at high levels, the hair roots induced by exogenous bacteria are considered as an appropriate option. In this experiment, five different strains of agrobacteriumn rhizogenes (CCTA15834, A13, A4, 9534, 318) used for induction of leaf and stock of water mint and studied the rooting and root growth in leaf samples (42/6%) and stock (50%). Among strains of bacteria, 15834 bacteria had the highest percentage of rooting and among the finest specimens, the stock was chosen as the best option for inducing hair roots. The emerged roots has grown in different environments (N+, P+, PH+, P- and CO+) and calculated secondary mextabolite of rozmarinic acid in these by high performance liquid chromatography (HCLP). The production of rosmarinic acid has increased in all environments as compared to the control environment .Among these, the environment containing phosphorus had the highest amount of rosmarinic acid (26647.027 ng⁄ml) Rosemaric acid production increased in all environments than control environment. Meanwhile, the environment containing phosphorus had the highest amount of rosemarinic acid. In molecular studies section, DNA extracted from the hair roots to confirm the validity of the measures and performed PCR reaction by the nuclear genome and proved successful integration of T-DNA in the genome of the hair roots after electrophoresis of PCR products. Also, to investigate the control role of this gene in water mint and also the manipulation of the rosmarinic acid biosynthesis pathway, the smMyB39 gene was isolated, matched and sequenced from the water mint. Finally, by verifying smMYB39 inhibitor homolog genes has proven the exxpression of Rosmarine Acid by semi-quantized PCR and the effect of the elicitors on the regulating genes of this mextabolite in vitro. The results of the mean comparison of the data indicates changes in the growth medium of the hairy roots caused to increase in the exxpression of the semi-quantitative exxpression of the smMYB39 gene. On the other hand, the amount of rosmarinic acid production has increased in these environments. According to the studied papers, it was expected that as the amount of mextamorphic rosemary production increased, the target gene exxpression would be lower, but the results suggest contrary to this.