Abstarct: One of the basic concepts of sustainable agriculture is the use of organic and biological fertilizers with the aim of eliminating or reducing the use of chemical inputs. To evaluate the effects of fungi’s compost (SMC), arbuscular mycorrhizal fungi and nitrogen fertilizer on some of soil’s characteristics in cowpea, an experiment was design as a factorial split plot baxse sign on complete randomized block. agriculture college in shahrood university in 2016. Treatments consisted of nitrogen fertilizer at two levels (0 and 100kg/ha) as a main factor, SMC at three levels (0, 10 and 20 ton.ha-1) and arbuscular mycorrhizal fungus at two levels (non-inoculated and inoculated) were considered as sub plots. The main effect of SMC on most traits measured in this research included: Percentage of mycorrhizal colonization, soil microbial respiration, leaf dry weight, stem dry weight, number of seeds per pod, main stem height, leaf area index, chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, soil phosphorus and protein content were sign meaningful. The combined application of SMC and mycorrhizal fungus had a positive and significant effect on leaf dry weight gain, grain yield, 100 seed weight, main stem height, leaf area index and harvest index. The interaction between nitrogen fertilizer and SMC significantly increased the percentage of mycorrhizal colonization, soil microbial respiration, leaf dry weight, chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, soil phosphorus and protein content. Generally, baxsed on this study, interaction of nitrogen fertilizer SMC mycorrhizal fungi can be considered as the best treatment for stem dry weight, seed number per pod, grain yield, chlorophyll b, carotenoids, leaf area index , Grain protein and harvest index.