Abstarct: As a typical climacteric fruit, fresh tomato is a perishable vegetable which senesces after harvest at ambient temperature. Ethylene plays a crucial role in the process of the ripening and senescence of tomato fruit. This study was conducted in order to investigate the effect of 1-MCP and film (Packing) on some characters of two tomato lines (Yellow pear beams and Orange beery) and evaluates the exxpression profiling of few genes involved in the ethylene biosynthesis. In the current study, tomatoes at the stage of colour defect from field were harvested. Experiment was done as a factorial design in a completely randomized design. The first factor was two tomato lines, the second factor included film, film with 1-MCP and control and third factor was four time points after harvesting. The assay was performed in three replications and tomato was kept at 20oC during 16 days storing period. The obtained results showed that there were significant differences between control treatment and other mentioned ones in terms of weight loss, pH, soluble solids and firmness. Regarding to weight loss, treatment with film was more effective than 1-MCP with film. However, the treatment with 1-MCP with film functions better than in other measured character. In gene exxpression profiling section, gene exxpression patterns of four genes (ACS4, ACO5, ACO1 and SAM1) involved in ethylene biosynthesis were investigated. The gained results demonstrated that the film and film with 1-MCP treatments reduced the exxpression level of mentioned genes compared with that of control. In total, the results showed that the film and film with 1-MCP treatments delayed the processes of the ripening and senescence of tomato fruit.