Abstarct: The investigation explored the associations among 14 agro-morphological traits and 21 ISSR amplified informative bands in a germplasm collection of 26 linseed by multiple linear regression analysis (MLR). Out of 258 ISSR amplified bands, 218 bands were polymorphic. The average number of polymorphic bands was 10.38 for each primer. The polymorphic information content (PIC) of the primers varied in the range of 0.29 and 0.49. The primers PCT6, UBC811, UBC815, IP3, PCT2, 3PCT5L, and 3PCT2 exhibited the highest PIC. There were significant relationships between some of the polymorphic bands and at least one of the 14 studied traits. The primers 3PCT2, 3PCT5L, IP5, 3PCT6, and UBC811 amplified more fragments related to various traits than other primers. The fragment generated by 3PCT5L with the size of 1200 bp was correlated with the number of branches and capsules per plant, capsule diameter, and seed yield per plant. This can be associated with the pliotropic impacts of this locus in controlling yield and some of its components. The amplified fragments with high coefficients of determination for each agro-morphological trait can be used, in addition to the mapping of the traits, in marker-assisted breeding programs after converting to SCAR marker, to select the best parents for the production of hybrids of linseed for which little genetic information is available.