Abstarct: Achieving sustainable soil fertility plays an important role in the development of agriculture and preventing the degradation of agricultural ecosystems and the ecological balance in the soil. Application of soil amendments such as phosphate dissolving bacteria and arbuscular mycorrhiza fungi can be an effective step in achieving optimum performance along with optimum use of inputs such as water and chemical fertilizers to achieve sustainable management of soil. In addition sustainable management, with considering the ecological principles, can create a balance in the environment and also increase the resource usage efficiency and provide a context for long-term use by human beings. Considering special conditions of pistachio orchards in terms of soil and lack of proper fertilizer management, the need for research on various aspects of the impact and use of biological fertilizers in the orchards is revealed. This study was conducted to evaluate the effect of mycorrhiza and phosphate 2 bio-fertilizer in different levels of phosphorus fertilizer on the growth of pistachio seedling. A factorial experiment was conducted in the form of blocks with a completely randomized design with three replications in “Porbaar Kesht –e- Damqan” company. Factors examined include mycorrhiza fungi on three levels, M0, M1 and M2, 2 bio- fertilizer in two levels, inoculated (B0) and none-inoculated (B1) and triple super phosphate fertilizer in three levels of p0, p1, p2. The results indicated that both separate and joint application of mycorrhiza 2 bio- fertilizer significantly effected some morphologic attributes such as height, shoot dry weight and root length. Mycorrhiza and 2 bio- fertilizer interactions significantly effected plant’s phosphorus concentration, plant’s potassium, soluble nitrogen and soil’s dissolved phosphorus. The results of this study also indicate that the application of M1 fungi (Glomous intraradices) in pot and phosphorus control level (zero) had the most impact in terms of pistachio seedlings colonization. It seems that the use of mycorrhiza fungi along with phosphate 2 bio-fertilizer can be replace with a part of chemical phosphorus fertilizers in pistachio orchards.