Abstarct: Agro-ecosystem are able to increase greenhouse gases, particularly carbon dioxide.The soil organic carbon is considered as the most important land carbon resorce. That's why any management practices on soil carbon dioxide, can be effective in increasing and decreasing it. In order to determine the effects of tillage on soil organic carbon under organic and chemical fertilizer application, an experiment was conducted in Shahrood university research farm. The experiment was a split plot factorical in a randomized complete block design with there replication. Factor were tillage with 2 level (conventional and conservation tillage), chemical fertilizer with 3 levels: common use, 50% of common use and 25% of common use (as control) and organic fertilizer as vermicompost with 3 levels (0, 5 and 10 ton/ha). The results showed that the main effect of vermicompost and fertilizer significantly increased grain protein, phosphorus and total nitrogen compared to control. Application of vermicompost increased soil respiration significantly compared to control. Also in semi-deep tillage an increase of soil respiration rate was observed. Also the effects of tillage × fertilizer × vermicompost was significantly effect on the amount of organic carbon and organic matter and soil carbon sequestration. So that semi-deep tillage × chemical fertilizer commonly × 10 tn/ha vermicompost coused 32.74% increase carbon sequestration in soils compared to the control. According to the experiment, the effect of chemical fertilizer (commonly) × 10 tn/ha vermicompost was the best treatment for increase of soil organic carbon, organic matter and carbon sequestration in soil and also grain yield and biologic yield. An increase in soil respiration was observed in semi-deep tillage. So it seems that combined use of chemical fertilizer and vermicompost as well as semi-deep tillage is a good strategy for reducing CO2 emissions into the atmosphere and increased carbon storage in soils. As a result, it seems that by reducing tillage and use of organic inputs such as vermicompost alone and combined with the chemical fertilizer, the invironmental impact caused by greenhouse gases via agro-ecosystems reduced.